摘要锟斤拷锟饺帮拷锟斤拷锟斤拷U锟斤拷锟斤拷锟斤拷锟斤拷锟角的攝取锟斤拷要通锟絕锟斤拷锟斤拷锟斤拷锟角撅拷锟叫革拷锟紿锟斤拷锟斤拷(Km=0.35 mM)锟侥革拷露锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟斤拷酶系锟統(t锟斤拷ng)(PTS)锟斤拷锟絰锟斤拷锟杰夛拷锟斤拷锟斤拷2-脫锟斤拷锟斤拷锟斤拷锟斤拷(2DG)锟斤拷缺锟斤拷PEP锟斤拷賴锟斤拷锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓锟斤拷锟皆碉拷突變锟絯锟斤拷锟紷些突變锟絯锟杰夛拷锟斤拷锟斤拷锟斤拷锟斤拷锟角烇拷唯一碳源锟斤拷锟斤拷源锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟絃锟斤拷锟斤拷锟节诧拷锟矫碉拷锟角碉拷锟紿锟斤拷锟斤拷(Ks=11 mM)锟侥凤拷PTS锟絲取系锟統(t锟斤拷ng)锟斤拷锟斤拷锟皆攝锟斤拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟节诧拷同锟紹透锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟絃锟斤拷野锟斤拷锟酵硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟绞和憋拷锟斤拷锟絃锟斤拷锟绞斤拷锟斤拷锟斤拷锟斤拷(d锟斤拷ng)锟紷些锟斤拷锟紹(y锟斤拷ng)锟斤拷募锟�(x锟斤拷)锟斤拷锟节橈拷(bi锟斤拷o)锟斤拷(zh锟斤拷n)锟絣锟斤拷锟铰測锟斤拷锟絩锟紷示锟斤拷锟斤拷锟斤拷锟狡碉拷PTS锟斤拷锟皆★拷然锟斤拷锟斤拷锟斤拷(d锟斤拷ng)锟斤拷(x锟斤拷)锟斤拷锟斤拷锟铰懸革拷锟节诧拷同锟紹透锟斤拷锟侥撅拷锟絖液锟叫時锟斤拷PTS锟斤拷锟斤拷锟紷示锟絚锟紹透锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷锟紷锟絅锟斤拷锟斤拷效锟斤拷(y锟斤拷ng)锟杰夛拷锟斤拷锟节高滲透锟斤拷锟絣(f锟斤拷)锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟絕锟届到锟斤拷锟斤拷锟斤拷锟秸猴拷锟斤拷锟絃锟斤拷锟斤拷锟铰斤拷锟斤拷原锟斤拷锟斤拷锟斤拷锟角凤拷PTS锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓系锟統(t锟斤拷ng)锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紹透锟斤拷锟斤拷影锟�锟斤拷锟节癸拷锟絀(y锟斤拷)锟斤拷锟絘(ch锟斤拷n)锟絣锟斤拷锟斤拷锟絊锟斤拷锟饺帮拷锟斤拷e锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紹透锟斤拷锟斤拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟絕锟届到锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�锟斤拷锟紷锟絅锟紽(xi锟斤拷n)锟斤拷亩锟斤拷锟斤拷锟斤拷税l(f锟斤拷)锟斤拷锟絕锟教ｏ拷锟斤拷锟斤拷锟斤拷锟绞的斤拷锟酵曪拷锟斤拷(d锟斤拷o)锟铰硷拷(x锟斤拷)锟斤拷锟絊锟街讹拷锟斤拷锟侥碉拷锟角憋拷锟斤拷锟斤拷锟斤拷锟斤拷同锟絩锟斤拷锟絊锟斤拷锟絘(ch锟斤拷n)品锟絘(ch锟斤拷n)锟绞的斤拷锟酵★拷

锟絇(gu锟斤拷n)锟絀锟絶锟斤拷锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓锟斤拷锟紹透锟斤拷锟斤拷锟饺帮拷锟斤拷l(f锟斤拷)锟斤拷锟斤拷锟饺帮拷锟斤拷锟斤拷U锟斤拷锟斤拷

锟叫撅拷锟斤拷(n锟斤拷i)锟捷ｏ拷

1锟斤拷锟紹透锟斤拷锟斤拷PTS锟斤拷锟斤拷锟角攝取系锟統(t锟斤拷ng)锟斤拷锟斤拷锟斤拷影锟�

锟紹透锟斤拷800mOsmol/Kg锟皆冿拷(n锟斤拷i)PTS锟斤拷锟皆維锟街恒定锟斤拷之锟斤拷锟絊锟斤拷锟紹透锟斤拷锟斤拷锟斤拷PTS锟斤拷锟皆撅拷锟斤拷锟铰斤拷锟斤拷锟紹透锟斤拷1900mOsmol/Kg锟絩PTS锟斤拷锟斤拷只锟斤拷锟斤拷邥r锟斤拷15%锟斤拷

2锟斤拷锟紹透锟斤拷锟斤拷锟饺帮拷锟斤拷l(f锟斤拷)锟酵碉拷影锟�

锟饺帮拷锟斤拷痛渭锟斤拷锟斤拷x锟絘(ch锟斤拷n)锟斤拷姆e锟桔曪拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紹透锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟剿維锟斤拷pH值锟姐定锟斤拷锟紸(锟节憋拷锟斤拷锟叫為氨)锟侥硷拷锟斤拷锟組一锟斤拷锟接勶拷锟剿滲透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟节谷帮拷锟斤拷锟斤拷锟経锟斤拷锟截讹拷锟斤拷锟叫撅拷锟斤拷2262锟斤拷锟絕锟届到锟紷一锟紽(xi锟斤拷n)锟斤拷該锟斤拷锟斤拷锟杰夛拷锟絖锟斤拷锟竭產(ch锟斤拷n)锟斤拷锟斤拷(锟饺帮拷锟斤拷>85g/L)锟斤拷锟揭滲透锟斤拷锟斤拷锟絕2000 mOsmol锟斤拷kg-1锟斤拷锟斤拷锟斤拷锟斤拷讉锟叫★拷r锟斤拷(n锟斤拷i)锟角碉拷锟斤拷锟斤拷锟绞憋拷锟街诧拷變锟斤拷然锟斤拷锟斤拷u锟铰斤拷锟斤拷锟斤拷锟剿檢锟斤拷锟皆減锟斤拷锟角凤拷锟絚锟饺帮拷锟斤拷锟桔積锟斤拷(d锟斤拷o)锟铰的滲透锟斤拷锟斤拷锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷锟統锟斤拷锟斤拷PTS锟斤拷锟皆岋拷锟斤拷锟角攝取锟斤拷锟斤拷貢锟絀锟斤拷锟斤拷野锟斤拷锟斤拷锟饺帮拷锟斤拷锟斤拷锟経锟斤拷ATCC 17965锟叫測锟斤拷锟斤拷貢锟絀一锟斤拷锟斤拷锟斤拷4.5 mmol锟斤拷g-1锟斤拷h-1锟斤拷锟节谷帮拷锟斤拷锟斤拷锟絘(ch锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟角攝取锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紹透酶锟斤拷锟皆革拷锟斤拷锟斤拷锟节凤拷锟絳锟饺條锟斤拷锟铰測锟矫碉拷锟斤拷锟斤拷锟角攝取锟斤拷锟绞憋拷锟斤拷锟节谷帮拷锟斤拷锟斤拷锟経锟斤拷2262锟叫滲透酶貢锟絀锟斤拷锟絖25%锟侥匡拷锟斤拷锟斤拷锟角攝取锟斤拷锟斤拷锟斤拷锟斤拷PTS锟紻(zhu锟斤拷n)锟絓系锟統(t锟斤拷ng)锟斤拷锟絯锟斤拷y锟斤拷锟斤拷锟斤拷锟統锟斤拷锟紹透锟斤拷(y锟斤拷ng)锟斤拷锟斤拷PTS锟斤拷锟皆碉拷锟斤拷锟斤拷影锟�锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟较條锟斤拷锟斤拷(锟紹透酶锟斤拷貢锟絀锟斤拷锟皆猴拷锟皆诧拷計)(锟酵氾拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小锟斤拷2g锟斤拷l-1)锟斤拷锟斤拷锟皆匡拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟铰斤拷锟絚锟紸(y锟斤拷)锟統锟斤拷PTS锟斤拷锟斤拷锟铰斤拷锟角筹拷锟接斤拷(锟紻3a)锟斤拷锟斤拷(d锟斤拷ng)锟斤拷锟矫維锟斤拷锟絕锟竭濓拷鹊锟斤拷锟斤拷锟斤拷锟斤拷a锟较诧拷锟皆ｏ拷50 g锟斤拷l-1锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫革拷锟狡匡拷锟斤拷锟斤拷锟斤拷锟节滲透酶锟斤拷锟皆和凤拷锟斤拷(y锟斤拷ng)锟斤拷锟叫达拷锟节碉拷锟斤拷锟斤拷锟角岋拷該系锟統(t锟斤拷ng)锟斤拷飽和度革拷(锟紻3b)锟斤拷锟斤拷锟紷锟絅锟絣锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟绞岋拷(d锟斤拷o)锟斤拷锟絶锟斤拷锟斤拷x锟斤拷锟斤拷^锟斤拷锟斤拷F(xi锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷

3锟斤拷锟斤拷锟饺帮拷锟斤拷a(ch锟斤拷n)锟绞碉拷影锟�

一锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟叫的滲透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絰锟斤拷锟斤拷锟铰斤拷锟侥猴拷锟街伙拷前l(f锟斤拷)锟酵結(ji锟斤拷)锟斤拷锟絩锟饺帮拷锟斤拷a(ch锟斤拷n)锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟绞碉拷锟铰斤拷锟斤拷锟斤拷锟斤拷劝锟斤拷锟絘(ch锟斤拷n)锟绞碉拷锟铰斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟斤拷锟斤拷CO2锟斤拷锟斤拷锟斤拷锟角憋拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘(ch锟斤拷n)锟紸锟斤拷CO2锟絘(ch锟斤拷n)锟斤拷锟斤拷锟铰憋拷锟街恒定锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟角分斤拷锟斤拷x锟叫獲锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷M锟姐細(x锟斤拷)锟斤拷锟絊锟街碉拷锟斤拷锟斤拷愣�锟斤拷锟紷锟絅锟斤拷锟斤拷锟絚锟絣(f锟斤拷)锟酵罐冿拷(n锟斤拷i)锟斤拷锟斤拷锟斤拷锟斤拷锟缴憋拷锟斤拷锟斤拷锟斤拷锟斤拷潜锟斤拷锟斤拷锟斤拷锟斤拷实慕锟斤拷锟斤拷锟揭拷锟斤拷锟斤拷锟斤拷锟斤拷堑谋锟斤拷锟�锟斤拷锟斤拷(d锟斤拷ng)使锟矫谷帮拷锟斤拷锟斤拷锟経锟斤拷锟侥維锟斤拷系锟斤拷(sh锟斤拷)0.6mmol锟斤拷锟斤拷锟斤拷/g锟斤拷(x锟斤拷)锟斤拷/h計锟斤拷锟斤拷锟斤拷锟节硷拷(x锟斤拷)锟斤拷锟絊锟斤拷锟斤拷要锟斤拷锟斤拷锟斤拷锟角的憋拷锟斤拷锟侥谷帮拷锟斤拷锟斤拷锟絘(ch锟斤拷n)锟侥筹拷始锟紸锟轿匡拷锟斤拷锟斤拷锟侥碉拷15%锟斤拷锟接碉拷锟絣(f锟斤拷)锟酵猴拷锟节滲透锟斤拷锟斤拷邥r锟斤拷40%(锟紻4)锟斤拷锟节發(f锟斤拷)锟酵猴拷锟斤拷锟絕锟届到锟侥谷帮拷锟斤拷a(ch锟斤拷n)锟斤拷锟铰斤拷锟絚锟絊锟斤拷锟斤拷锟斤拷锟教硷拷锟斤拷x锟斤拷锟斤拷變锟斤拷一锟铰★拷锟斤拷锟斤拷(j锟斤拷)锟斤拷锟絰锟斤拷锟皆減锟斤拷锟斤拷锟絞锟斤拷锟絰锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絫锟斤拷锟斤拷(j锟斤拷)锟斤拷變锟斤拷锟斤拷锟狡氾拷?zh锟斤拷)菨锟饺匡拷锟杰恢�?f锟斤拷)锟斤拷锟街產(ch锟斤拷n)锟绞損失锟斤拷

4.討論

锟斤拷锟斤拷锟絝锟斤拷锟剿谷帮拷锟斤拷l(f锟斤拷)锟酵猴拷锟节碉拷锟斤拷锟斤拷锟絚锟絘(ch锟斤拷n)锟斤拷e锟桔和滲透锟絟(hu锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥匡拷锟斤拷影锟街憋拷锟斤拷锟斤拷P(gu锟斤拷n)锟斤拷锟紷锟絅锟紽(xi锟斤拷n)锟斤拷锟斤拷锟斤拷锟节伙拷锟斤拷锟斤拷_水平锟斤拷變锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟节滲透锟絳锟饺條锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓系锟統(t锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟皆發(f锟斤拷)锟斤拷锟剿革拷變锟斤拷锟斤拷锟斤拷锟斤拷锟紷锟絅效锟斤拷(y锟斤拷ng)锟角匡拷锟斤拷锟�锟斤拷锟斤拷锟揭伙拷锟斤拷B透锟絳锟饺憋拷锟斤拷锟斤拷锟斤拷锟斤拷洗锟斤拷募锟�(x锟斤拷)锟斤拷锟斤拷锟杰恢忥拷(f锟斤拷)锟斤拷锟紻(zhu锟斤拷n)锟絓锟角碉拷锟斤拷锟斤拷锟斤拷同锟接ｏ拷锟斤拷(d锟斤拷ng)锟截戯拷锟节高滲锟侥撅拷锟絖液锟叫時锟斤拷锟斤拷锟斤拷锟斤拷锟絃锟侥硷拷(x锟斤拷)锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絓锟斤拷锟斤拷也锟紷锟斤拷锟斤拷锟斤拷锟斤拷

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锟斤拷锟斤拷效锟斤拷锟斤拷锟斤拷俚锟斤拷锟斤拷a(ch锟斤拷n)锟饺帮拷锟斤拷木锟斤拷锟斤拷_锟斤拷锟紹透锟街碉拷锟斤拷锟斤拷锟�锟斤拷锟紷锟斤拷锟斤拷(d锟斤拷o)锟铰發(f锟斤拷)锟斤拷24小锟絩锟斤拷锟斤拷墚a(ch锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟�锟斤拷锟斤拷锟�锟斤拷锟結锟絰锟斤拷锟叫革拷锟酵夛拷锟斤拷锟斤拷锟侥撅拷锟疥對锟絣(f锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟�锟斤拷锟侥讹拷锟絖锟斤拷锟斤拷锟絀(y锟斤拷)锟斤拷锟斤拷锟斤拷蚪锟�锟斤拷锟节獲锟矫高產(ch锟斤拷n)锟斤拷a(ch锟斤拷n)锟斤拷锟絩锟杰維锟街高达拷锟絰锟斤拷锟皆★拷锟斤拷一锟絣锟剿凤拷锟紷锟絅锟斤拷锟絰锟斤拷锟狡碉拷路锟斤拷锟絫锟斤拷锟絖锟絣(f锟斤拷)一锟絅锟絣(f锟斤拷)锟斤拷系锟統(t锟斤拷ng)锟斤拷锟杰夛拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷取锟絕锟斤拷锟节發(f锟斤拷)锟酵積锟桔時去锟斤拷锟絘(ch锟斤拷n)锟斤。